| Info. | Vol.1 - No.2 (2007.06.20) |
|---|---|
| Title | Surface Plasmon Resonance Biosensor Chips |
| Authors | Moonil Kim1, Sang Hee Han1,2, Yong-Beom Shin1 & Bong Hyun Chung1 |
| Institutions | 1BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea. 2University of Science and Technology (UST), Yuseong, Daejeon 305-333, Korea Correspondence and requests for materials should be addressed to B.H. Chung (chungbh@kribb.re.kr) |
| Abstract | In this study, we describe biosensors predicated on surface plasmon resonance (SPR), a phenomenon that has recently become a focus of interest in biomedical science research. SPR has been shown to occur on two-dimensional metal surfaces, which support the SPR-active substrate on a glass prism. The SPR biosensor, which exploits the SPR effect, is a label-free and surface-sensitive spectroscopic system that utilizes measured changes in the local refraction index upon adsorption. Based on the SPR phenomenon, other technologies have been developed, including SPR-MS, SPR microscopy, localized SPR, and SPR imaging. The development of an SPR imaging system has already begun to overcome one of the difficulties inherent to conventional SPR, namely its high-throughput limitation. In this review, the detection principles and properties of SPR biosensors are briefly elucidated, and the possible future biomedical applications of SPR biosensors, including high-throughput screening in drug discovery, analysis of biomolecular interactions, and proteomics research are also discussed. This discussion is particularly salient now that SPR imaging provides another useful tool for the performance of high-throughput assays. |
| Keyword | Surface plasmon resonance, SPR biosensor, SPR imaging, Gold chip, Label-free, Biomolecular interaction analysis |
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