| Info. | Vol.3 - No.4 (2009.12.20) |
|---|---|
| Title | Expression Profiling of Neuroinflammatory Genes in LPS and LPS/IFN款-Stimulated BV-2 Microglia Cells |
| Authors | In Su Kim1, Ji-Hwan Park1, Jin Hwan Do2, Hyun Myung Ko1, Eun Jung Park3, Kyoungho Suk4 & Dong-Kug Choi1 |
| Institutions | 1Department of Biotechnology, Konkuk University, Cheongju 380-701, Korea 2Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan 3Immune and Cell Therapy Branch, National Cancer Center, Goyang 410-769, Korea 4Department of Pharmacology, Kyungpook National University School of Medicine, Daegu 700-422, Korea Correspondence and requests for materials should be addressed to D.-K. Choi (choidk@kku.ac.kr) |
| Abstract | A large body of evidence indicates that microglia, resident innate immune cells in the brain, play a role in host defense and function as active contributors to neuron damage in neurodegenerative disease. Microglia are crucial in the immune response within the central nervous system (CNS), and function in protecting the brain against injury. In contrast, activated microglia can secrete numerous reactants that damage neurons. The pathogenesis of various neurodegenerative diseases has been associated with microglia activation. Elucidation of molecular mechanisms underlying microglia activation could aid in controlling neuroinflammation and subsequent neurodegeneration. To increase the understanding of microglia activation, a cDNA microarray was presently used to profile genome-wide expression in activated BV-2 microglia cells. The analysis revealed that genome expression was differentially modulated for 1.2% of the total cellular transcripts in lipopolysaccharide/interferon-款 (LPS/IFN款)-activated BV-2 microglia cells. Comparison of array data between LPS- and LPS/IFN款-activated cells showed that the transcriptional signature was similarly regulated. This common expression pattern of stimulation was statistically significant, with>2-fold change evident for 38 genes. To evaluate the functional significance of the altered gene expression in microglia cells, PBEF1, an induced gene both LPS and LPS/IFN款-activated BV-2 microglia, was further analyzed in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-intoxicated animal model. |
| Keyword | Neuroinflammation, Microglia, Microarray analysis, Interferon gamma, Lipopolysaccharide |
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