| Info. | Vol.3 - No.4 (2009.12.20) |
|---|---|
| Title | Gene Expression Pattern Analysis of PAHs and VOCs in Rat Blood Using HazChem Rat Array |
| Authors | Hye-Won Park1,2, Seung-Jun Kim1,3, Moon-Ju Oh1, Seung-Jun Wang6, Hyunseok Cho3, Chansoo Park2, JaeRyul Han3, Youn-Jung Kim5, Suk Woo Nam4, Jae-Chun Ryu5 & Seung Yong Hwang1,3 |
| Institutions | 1Genocheck Co., Ltd., Sa 1-dong, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea 2Department of Bio-Nanotechnology, Hanyang University, Sa 1-dong, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea 3Department of Biochemistry, Hanyang University, Sa 1-dong, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea 4Department of Pathology, Microdissection Genomics Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea 5Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology, PO Box 131, Cheongryang, Seoul 130-650, Korea 6Environmetal Epidemiology Division, Environmental Health Risk Research Department, National Institute of Environmental Research, Gyeongseo-dong, Seo-gu, Incheon 404-708, Korea Correspondence and requests for materials should be addressed to S.Y. Hwang (syhwang@hanyang.ac.kr) |
| Abstract | The HazChem rat array includes 233 VOCs- and PAHs-specific genes in rat blood samples, 445 VOCsor PAHs- or POPs-differentially-expressed genes in rat in vivo samples and 847 liver-toxicity-related genes in previous studies. In previous experiments, the expression levels of these genes were altered in whole genome microarray experiments using VOCtreated and PAH-treated rat blood cells. In this study, we employed the rat HazChem array to evaluate the gene expression patterns of the chemical groups. The chemical groups utilized in this study were PAHs and VOCs. We employed benzo[a]anthracene and benzo [a]pyrene as examples of PAHs, and dichloromethane, ethylbenzene, and trichloroethylene as examples of VOCs. Thus, a total of 5 chemicals were ultimately evaluated. We selected 239 genes that evidenced significant alterations in expression with 5 environmental toxicants. We also verified that the 5 chemicals utilized herein were divided into 2 chemical groups on the basis of the 62 genes. We also selected 117 commonly expressed genes associated with all types of chemicals. In the gene ontological data, these genes were associated with responses to stress, macromolecular biosynthesis, catabolism, cell adhesion, response to endogenous stimulus, etc. Thus, the results of this study show that the HazChem rat array can be used to predict the toxicity of chemical species, as well as the effects of environmental toxicants. |
| Keyword | Toxicogenomics, PAHs (Polycyclic Aromatic Hydrocarbons), VOCs (Volatile Organic Compounds), Microarray, Environmental hazards |
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