| Info. | Vol.4 - No.3 (2010.09.20) |
|---|---|
| Title | Comparison of the performance of the PANArray??HPV test and DNA chip test for genotyping of human papillomavirus in cervical swabs |
| Authors | Hye Jong Song 1, Jeong Won Lee2, Byoung Gie Kim 2, Sang Yong Song 1, Duk Soo Bae 2 & Dae Shick Kim 1 |
| Institutions | 1Department of Pathology, Samsung Medical Center,
Sungkyunkwan University School of Medicine, Seoul 135-710, Korea 2Department of Obstetrics and Gynecology, Samsung Medical Center Sungkyunkwan University School of Medicine, Seoul 135-710, Korea Correspondence and requests for materials should be addressed to D.S. Kim (oncorkim@skku.edu) |
| Abstract | The aim of this study was to evaluate the recently developed PANArray??Human papilloma virus (HPV) kit for detection and genotyping of 19 high-risk and 13 low-risk HPV types, and compare it with the commercially available DNA chip kit geno-typing of 24 HPVs. We llected cervical swabs from 741 patients with various stages of invasive cervical carcinoma being treated at the Samsung Medical Center. The overall HPV positivity rate was 73% using PANArray??HPV and 72.1% with the DNA chip, and no statistically significant differences were found with respect to the cytology grade. Comparing the results of the two chips, concordant results were found in 637/741 samples (85.9%), compatible in 69/741(9.3%) and discordant in 35/741(4.7%). Type-specific sequencing analysis of all samples revealed a 99.7% confirmation of PANArray??HPV genotyping results, compared to 91.0% of DNA chip. The PANArray??HPV test thus proved to be highly sensitive and accurate even when multiple HPV infections were present. |
| Keyword | PNA, PNA microarray, Human papillomavirus (HPV), HPV genotyping |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
