| Info. | Vol.4 - No.3 (2010.09.20) |
|---|---|
| Title | Biomarker analysis of rat livers exposed to different toxic pollutants (VOCs and PAHs) using an antibody array |
| Authors | Eung-Yoon Kim1 , Mi-Young Lee2 , Seung Yong Hwang3 & In-Cheol Kang1 |
| Institutions | 1Department of Biological Science and BioChip Research Center,
Hoseo University & Innopharmascreen Inc., Asan 336-795, Korea 2Department of Medicinal Biotechnology, SoonChunHyang University, Asan 336-745, Korea 3Division of Molecular and Life Science, Hanyang University & Geno Check Co. Ltd., Sangnok-gu, Ansan, Gyeonggi-do, Korea Correspondence and requests for materials should be addressed to I.-C. Kang (ickang@hoseo.edu) |
| Abstract | We have developed an antibody microarray chip for biomarker analysis in rat livers exposed to five different toxic pollutants: volatile organic compounds (VOCs), dichloromethane, ethylbenzene, and trichloroethylene; and polycyclic aromatic hydrocarbons (PAHs), benzoanthracene and phenanthrene. The antibody chip consisted of antibodies against different biomarkers: alpha-2 關 globulin, glutathione S transferase (GST)-慣, Hemoglobin 棺, and Phenylalanine hydroxylase. VOC- and PAH-treated rat livers showed differential expression patterns of the biomarker proteins. These results demonstrate that a protein chip based antibody array is useful for the analysis of biomarkers induced by environmental toxic pollutants and can be a powerful tool to study the mechanisms of pollutants in biological samples. |
| Keyword | Protein chip, Biomarker assay, Environmen-tal pollutants, Antibody array, VOCs, PAHs |
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