| Info. | Vol.5 - No.2 (2011.06.20) |
|---|---|
| Title | Microarray of stimuli-responsive microbeads for duplexed immunoassay |
| Authors | Jonghwan Lee1, Jaeyeon Jung1, Subeom Park1, Jie Chen1, Jaeyoo Choi1 & Jinho Hyun1 |
| Institutions | 1Department of Biosystems and Biomaterials Science and Engineering,1Seoul National University, Seoul 151-742, Korea Correspondence and requests for materials should be addressed to J. Hyun (jhyun@snu.ac.kr) |
| Abstract | This paper describes a duplexed immunoassay method based on a functional microbeads (關beads) array. For selective detection of dual analytes, the 關beads were conjugated with a responsive biopolymer, characterized by a different phase transition temperature. Then, the 關beads modified with elastin-like polypeptides (ELPs) were spatially localized in a single microwell through sequential injection with a buffer. Prostate specific antigen (PSA) and mouse immunoglobulin G (IgG) were selected to show the feasibility of duplexed immunoassay. The detection limit of the 關bead array with PSA and mouse IgG was 0.05 ng/mL and 0.1 ng/mL, respectively. |
| Keyword | Microbead, Spatially controlled microarray, Duplexed immunoassay, Phase transition, Antibody array |
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