| Info. | Vol.5 - No.2 (2011.06.20) |
|---|---|
| Title | Comparison of genomic profiles in human neuroblastic SH-SY5Y and substrate-adherent SH-EP cells using array comparative genomic hybridization |
| Authors | Jin Hwan Do1, In Su Kim2, Jae Dong Lee3 & Dong-Kug Choi2 |
| Institutions | 1Bio-food and Drug Research Center, Konkuk University, Chungju 380-701, Korea 2Department of Biotechnology, Konkuk University, Chungju 380-701, Korea 3Department of Internal Medicine, School of Medicine, Konkuk University Chungju Hospital, Chungju 380-704, Korea Correspondence and requests for materials should be addressed to D.-K. Choi (choidk@kku.ac.kr) |
| Abstract | Human neuroblastoma is the most commonly diagnosed solid tumor in children. The existence and development of S-type cells is important for the prognosis and malignant properties in neuroblastoma patients. However, their origin is controversial and the relationship between S- and N-type cells in neuroblastoma has not yet been clarified. To investigate the feasibility of inter-conversion and characteristic features between S-type cells lacking malignant potential and N-type cells having metastatic potential, the genomic profiles of neuroblastoma SH-EP (S-type) and SH-SY5Y (N-type) cells were compared at high resolution. Common gain segments (??0 Mb) between SH-EP and SH-SY5Y cells were observed at 1q21.1- q44 and 17q21.32-q25.3. The results of the fluorescent in situ hybridization (FISH) analysis showed good agreement with the array-CGH data. Genome-wide inspection of SH-EP and SH-SY5Y cells successfully identified not only common chromosomal aberrations but also genomic variations, suggesting that interconversion could not take place between S- and Ntype cells. The identified differences between less aggressive S-type cells (SH-EP) and highly aggressive neuroblastic N-type cells (SH-SY5Y) might be useful for understanding tumorigenicity and discovery of potential new markers in neuroblastoma. This is the first effort to compare genomic profiles between less aggressive S-type cells (SH-EP) and highly aggressive neuroblastic N-type cells (SH-SY5Y) at high resolution. |
| Keyword | Copy number variation, Neuroblastoma SHEP and SH-SY5Y, Array-based comparative genomic hybridization (array-CGH), Inter-conversion |
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