| Info. | Vol.5 - No.3 (2011.09.20) |
|---|---|
| Title | Construction of branched DNA for SNP determination on glass-chip using photochemical ligation |
| Authors | Minhaz Uddin Ahmed1,2, Yoshinaga Yoshimura1, M. Mosharraf Hossain3, Eiichi Tamiya3 & Kenzo Fujimoto1 |
| Institutions | 1Japan Advanced Institute of Science and Technology (JAIST),
Asahidai, Nomi, Ishikawa, Japan 2Institut National de la Recherche Scientifique Centre-EMT, 1650 Boulevard Lionel-Boulet, Varennes (Quebec), J3X 1S2, Canada 3Department of Applied Physics, Graduate School of Engineering, Osaka University 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan Correspondence and requests for materials should be addressed to M.U. Ahmed ( minhaz@emt.inrs.ca) |
| Abstract | Single nucleotide polymorphisms (SNPs) are the most frequent form of sequence variation among individuals. Besides, sequence specific DNA detection has been an issue for its application in the diagnosis of pathogenic and genetic diseases. Many detection techniques that rely upon target hybridization with radioactive, fluorescent, chemiluminescent, and other types of label probes have been developed so far. Chip based detection for analysis of biomedical features like SNPs is not widely used in routine clinical settings, because it needs lot of improvement in terms of reproducibility and fidelity. Here, we have shown SNP typing on glass chip using KRAS oncogene sequence as a model. We have also shown that this SNP typing can be multiplexed by the further optimization of the photoligation protocol for high throughput detection that may lead to determine the sequence selectivity, less restricted reaction conditions and high efficiency. |
| Keyword | Glass chip, Branched DNA, Photochemical ligation, SNP, ODN |
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