| Info. | Vol.5 - No.4 (2011.12.20) |
|---|---|
| Title | Human lactoferrin upregulates BCL-3 in the K562 erythroleukemia cell |
| Authors | Byungtak Kim1, Ho Gun Chang1,* & Sun Jung Kim1 |
| Institutions | 1Department of Life Science, Dongguk University-Seoul, Seoul 100-715, Korea
*Present address: Hanmi Pharmaceutical, Gyeonggi-do 445-913, Korea Correspondence and requests for materials should be addressed to S.J. Kim ( sunjungk@dongguk.edu) |
| Abstract | Lactoferrin (LF) plays pivotal roles in primary defense against microbial infection and other cellular processing including immuno-modulation and growth stimulation of cells. To understand the molecular mechanism of LF action we stably overexpressed LF in the K562 erythroleukemia cells and examined differential gene expression using a cDNA microarray. One of the genes that were highly upregulated in the LF-overexpressing cells was the I觀B family member B cell lymphoma-3 (BCL-3) that is implicated to promote B cell proliferation and chronic lymphocytic leukemia. Northern blot as well as RT-PCR analysis confirmed that BCL-3 was highly induced in LF-transfected cells. Furthermore, by treating the K562 cells with natural LF protein, we observed a significant increase of BCL-3 messages with the peak at 50 關g/mL and 2 h after treatment. These results suggest that LF signals to induce BCL-3 and thereby promotes B cell proliferation and survival. |
| Keyword | B cell, BCL-3, K562, Lactoferrin, Leukemia |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
