| Info. | Vol.6 - No.2 (2012.06.20) |
|---|---|
| Title | Identification and characterization of flowering repressor-related genes in Chinese cabbage |
| Authors | ChangKug Kim1,??/sup>, Yeon-Hee Lee2,??/sup>, Joon-Ki Hong2, DongSuk Park1, Mi-Kyoung Kim1, MinSeok Cho1, YongKab Kim3 & JangHo Hahn1 |
| Institutions | 1Genomics Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA), Suwon 441-707, Korea 2Bio-crop Development Division, National Academy of Agricultural Science (NAAS), RDA, Korea 3School of Electrical Information Communication Engineering, Wonkwang University, Iksan 570-749, Korea ??/sup>These authors contributed equally to this work. Correspondence and requests for materials should be addressed to J.H. Hahn (jhhahn@korea.kr) |
| Abstract | In this study, we used a five-step process to identify those genes most likely involved in flowering repression in the Chinese cabbage (Brassica rapa). We tested 6,275 candidate genes with 300K microarrays, which included specific gene expression profiles of FLOWERING LOCUS C (FLC) mutants and normal cultivars during five cold vernalization stages. From that, we identified 289 transcription factor genes and 59 pathway network genes associated with floweringrelated metabolism. Then we compared the 348 genes to 1,287 genes from Gene Ontology and Clusters of Orthologous Groups analyses, which use similar orthologs to categorize conserved genes. Those analyses revealed 10 hypothetical genes for B. rapa, which we verified by reverse transcription-polymerase chain reaction. The final selected genes most likely play regulatory roles in either B. rapa flowering time control or flowering repression during vernalization. While these final genes require further characterization and validation, our study illustrates the usefulness of a multi-layered screening method after initially identifying genes from microarrays. |
| Keyword | Chinese cabbage, Flowering repressor genes, FLC genes |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
