| Info. | Vol.6 - No.2 (2012.06.20) |
|---|---|
| Title | Lipopolysaccharide-mediated protein expression profiling on neuronal differentiated SH-SY5Y cells |
| Authors | Nando Dulal Das1, Mi Ran Choi1, Kyoung Hwa Jung1, Ji Hyun Park1, Hyung Tae Lee1, Seung Hyun Kim2 & Young Gyu Chai1 |
| Institutions | 1Division of Molecular & Life Science, Hanyang University, Ansan 426-791, Korea 2Hanyang Cell Therapy Center, College of Medicine, Hanyang University, Seoul 133-792, Korea Correspondence and requests for materials should be addressed to Y.G. Chai (ygchai@hanyang.ac.kr) |
| Abstract | Neuroinflammation can contribute to neuronal dysfunction, death and several neurodegenerative diseases, including Alzheimer?셲 disease, Parkinson?셲 disease, amyotrophic lateral sclerosis, and multiple sclerosis. Lipopolysaccharide (LPS)-induced neuroinflammation severely affects neurons and can contribute to neuronal dysfunction and degeneration by causing the release of inflammatory and neurotoxic factors. We evaluated the long-term effects of treating differentiated SH-SY5Y cells with LPS to mimic LPS-induced neuroinflammation. Using matrix assisted laser desorption ionization-time of flight mass spectrometry and MetaCore pathway analysis software (GeneGo), the proteomic expression profiles of differentiated SH-SY5Y cells after LPS treatment was studied to determine the inflammatory effects on the process of SH-SY5Y differentiation. Long-term LPS treatment resulted in the upregulation of phosphodiesterase 4B (PDE4B), slit robo GTPase (SRGAP2), transcription repressor E2F-6, vimentin, and 70 kDa heat shock protein 9 (Mortalin/HSPA9). Taken together, our results suggest that LPS-treated differentiation of SH-SY5Y cells can lend insight into the multiple pathways involved in neurological diseases. |
| Keyword | SH-SY5Y cells, Lipopolysaccharide, Meta-Core pathway analysis software (GeneGo), Neuroinflammation |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
