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(사)한국바이오칩학회 The Korean BioChip Society





BT+IT+NT융합시대의 리더 : 한국바이오칩학회

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Info. Vol.6 - No.3 (2012.09.20)
Title Harnessing gravitational, hydrodynamic and negative dielectrophoretic forces for higher throughput cell sorting
Authors Junghun Lee1, Youngwoong Kim1, David J. Beebe2& Byungkyu Kim1
Institutions 1School of Aerospace and Mechanical Engineering, Korea Aerospace University, Goyang, Gyeonggi-do 412-791, Korea
2Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin, Madison, WI, USA
Correspondence and requests for materials should be addressed to B. Kim (bkim@kau.ac.kr)
Abstract We present a negative dielectrophoretic (n-DEP) force based high throughput cell sorting system integrating a cantilever-type electrode array in a vertical macro channel to leverage gravity driven flow and eliminate the need for external pumps. The system comprises a macro-sized channel to increase throughput, a cantilever electrode array(L횞W횞H=150關m횞500關m횞10關m) to achieve n-DEP force and high throughput, and a flow regulator to precisely control hydrodynamic force. The hydrodynamic force and the n-DEP force acting on the target cell are evaluated theoretically. In addition, optimal separation conditions are investigated using computational models. Separation conditions are experimentally investigated based on simulation results. Finally, to demonstrate the separation performance of the sorting system, we performed the separation of the human breast cancer cells (MCF 7) from diluted red blood cells(RBCs) under conditions of low voltage(7Vp-p with 500kHz) and flow rate of 5關L∙min-1.The system can separate MCF 7 cell with 71% separation efficiency in case of the ratio of 1:60000(MCF 7:RBCs).
Keyword Negative dielectrophoresis(n-DEP), Gravity driven flow, Macro-sized channel, Separation, Cantilever-type electrode(CTE) array, High throughput sorting (HTS)
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