| Info. | Vol.1 - No.3 (2007.09.20) |
|---|---|
| Title | Nanopatterning Proteins with a Stamp Tip for Dip-Pen Nanolithography |
| Authors | Dong-Sik Choi1,†, Seong-Hun Yun1,†, Young-Chul An1, Myoung-Jae Lee1, Dong-Gu Kang2, Soo-Ik Chang2, Hong-Kyeong Kim1, Kyung-Min Kim1 & Jung-Hyurk Lim1 |
| Institutions | 1Department of Polymer Science and Technology, Chungju National University, Chungju 380-702, Korea 2Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea Correspondence and requests for materials should be addressed to J.-H. Lim (jhlim@cjnu.ac.kr) †These authors contributed equally to this work. |
| Abstract | The fabrication of a nano-porous polyoxazoline stamp tip for scanning probe nanolithography has been reported, and the patterning of nanometer scale high-molecular weight proteins at ultra-fast speed demonstrated using the stamp tip. A nanoporous polymer-coated silicon tip was constructed using ring opening polymerization of 2-methyl-2-oxazoline on the surface of the tip employing a simple solution polymerization method. Because polyoxazoline is a hydrophilic and biocompatible polymer, these tips can easily absorb and release water-based biomolecules via their pores. Using dip-pen nanolithography, the patterning of Human IgG and PSA proteins using these stamp tips has been demonstrated. The results showed that protein nano-patterns can be generated at least five times faster with this method than with a conventional silicon tip. |
| Keyword | Atomic force microscopy (AFM), Polyoxazoline, Protein nano-patterns, Dip-pen nanolithography (DPN), Self-assembled monolayers (SAMs) |
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