| Info. | Vol.7 - No.2 (2013.06.20) |
|---|---|
| Title | Aqueous extracts of Anemarrhena asphodeloides stimulate glucagon-like pepetide-1 secretion in enteroendocrine NCI-H716 cells |
| Authors | Kang-Hoon Kim1,??/sup>, Ki-Suk Kim1,??/sup>, Min Hee Shin1, Eun Gyeong Jang1, Eun Young Kim1, Jang-Hoon Lee1, Kwang Seok Ahn1, Jae-Young Um1 & Hyeung-Jin Jang1 |
| Institutions | 1College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul 130-701, Korea |
| Abstract | Anemarrhena asphodeloides (AA), a bitter taste herbal medicine, has been prescribed in traditional oriental medicine to treat diabetes mellitus. Here, AA was extracted and fractionated to investigate its effects on the stimulation of glucagon-like peptide-1 (GLP-1) secretion in enteroendocrine cells. GLP-1 is secreted from the human enteroendocrine L cells to the blood in response to ingested nutrients. Because GLP-1 increases glucose dependent insulin release, it is known as a therapeutic method for the treatment of type II diabetes mellitus. The human enteroendocrine L cell line NCI-H716 expresses various chemoreceptors including the G protein coupled receptor (GPCR). Previous studies suggested that, through the GPCR signaling pathway, the secretion of GLP-1 can be induced in NCI-H716. Accordingly, we studied the GLP-1 stimulation effect of the AA extract and its mode-of-action using the GLP-1 ELISA and microarray. Functional categorization of the microarray data confirmed up or down-regulated gene expressions associated with the GPCR signaling pathway. This study demonstrates that AA extracts have a scientific possibility as a GLP-1 stimulant and thus may have the potential to be a therapeutic herbal medicine for type II diabetes mellitus. |
| Keyword | Anemarrhena asphodeloides (AA), Glucagonlike peptide-1 (GLP-1), Enteroendocrine cell, NCI-H716, Type II diabetes mellitus, Herbal medicine |
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