| Info. | Vol.1 - No.4 (2007.12.20) |
|---|---|
| Title | Microbiochip for Electrical Detection of Carcinoembryonic Antigen Using Gold Nanoparticles and Silver Enhancement |
| Authors | Joon-Ho Maeng1, Yong-Jun Ko2, Yoomin Ahn3, Nahm-Gyoo Cho3, Seoung-Hwan Lee3 & Seung Yong Hwang4 |
| Institutions | 1Department of Biochemistry, Graduate School, Hanyang University, Ansan, Gyeonggi-do 426-791, Korea 2Department of Precision Mechanical Engineering, Graduate School, Hanyang University, Seoul 133-791, Korea 3Department of Mechanical Engineering, Hanyang University, Ansan, Gyeonggi-do 426-791, Korea 4Division of Molecular and Life Science, Hanyang University and GenoCheck Co. Ltd., Ansan, Gyeonggi-do 426-791, Korea Correspondence and requests for materials should be addressed to S.Y. Hwang (syhwang@hanyang.ac.kr) |
| Abstract | The conventional immunoassay protocol for the detection of carcinoembryonic antigen (CEA) is expensive, laborious, and time-intensive. Additionally, it requires a very large detection device. In an effort to solve these problems, we have asse-ssed the utility of a novel immunoassay technique based on microbeads and gold nanoparticles. In our microbiochip, we have adopted the filtration method to fix the microbeads, and used the immunogold silver staining (IGSS) method to amplify the electrical signal. The microbiochip was constructed of a Pyrex glass substrate and an inexpensive and biocompatible polydimethylsiloxane (PDMS) layer. The platinum (Pt) microelectrode for electrical signal detection was fabricated on the substrate. A microchannel and pillar- type microfilter was formed in the PDMS layer. The colon cancer marker, CEA, was assayed via a sandwich immunoassay. As a result, we obtained significant resistance signal as the difference in CEA concen-tration. The total analysis time was less than one hour. A bead-based electrical de-tection system, such as the one described in this study, can be applied to systems for cancer diagnosis. |
| Keyword | Carcinoembryonic antigen, Immunoassay, Microbiochip, Immunogold silver staining, Microfilter, Microbead |
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