| Info. | Vol.9 - No.1 (2015.03.20) |
|---|---|
| Title | Implantation of Encapsulated Human Septal Chondrocytes into Immunocompetent Mice Using Alginate Microfibers |
| Authors | Jung Hwal Shin1, Jin Hwa Jung1, Hyoryung Nam2, Sung Won Kim3, Dong-Woo Cho1 & Geunbae Lim1,* |
| Institutions | 1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongsangbuk-do 790-784, Korea 2Department of Integrative Bioscience and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongsangbuk-do 790-784, Korea 3Department of Otolaryngology- Head and Neck Surgery, The Catholic University of Korea, Seoul 137-701, Korea |
| Abstract | Cartilage regeneration is a major challenge for researchers because cartilage tissue has limited innate regenerative ability. Encapsulation within an alginate gel has been used widely for 3D scaffolds to generate cartilage-like tissue, but alginate gels have limitations such as poor mechanical properties. In this study, we fabricated alginate microfibers for human septal chondrocyte (HSC) encapsulation and identified the conditions that result in the optimal mechanical properties of the alginate microfibers. In vitro experiments showed that HSCs encapsulated within alginate microfibers maintained 짚90% viability for 7 days, and the 140 關m condition was more effective in terms of HSC proliferation than the 330 and 520 關m conditions. In vivo, HSCs differentiated gradually into cartilage tissue over 4 weeks in immunocompetent mice. Importantly, the alginate-encapsulated HSCs were isolated and protected from the host immune response despite xenograft implantation. |
| Keyword | Alginate microfibers, Human septal chondrocyte (HSC), Immunocompetent mice, Cartilage regeneration, Immune response |
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