| Info. | Vol.1 - No.1 (2007.03.20) |
|---|---|
| Title | SUMO(Small Ubiquitin-like Modifier) Chip for Analysis of SUMO-conjugation to a Target Protein |
| Authors | Young-Hee Oh1, Young-Pil Kim1 & Hak-Sung Kim1 |
| Institutions | 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea Correspondence and requests for materials should be addressed to H.S. Kim (hskim76@kaist.ac.kr) |
| Abstract | The analysis of protein interactions and modifications in a microarray format has become the focus of great interest in researchers in drug discovery, diagnostics, and cell biology, due to the advent of rapid and high-throughput biological assays requiring only a small amount of sample. Herein, we describe a chip-based analysis of sumoylation, the post translational modification (PTM) process involving the covalent attachment of the small ubiquitin-like modifier (SUMO) family protein to a target protein in a mammalian cell. The protein was expressed using an in vitro translation system and immobilized directly onto a glass slide using epitope tags fused to the protein. In this paper, the microarray-based analysis technique was shown to be a useful strategy for the identification of SUMO target proteins from preexisting protein pools and proteome arrays, in a highthroughput manner. |
| Keyword | PTM (post translational modification), SUMO (small ubiquitin-like modifier), Protein microarray, Cell-free expression system |
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