| Info. | Vol.10 - No.3 (2016.09.20) |
|---|---|
| Title | Development of a Matrix Metalloproteinase-2 (MMP-2) Biosensing System by Integrating an Enzyme-mediated Color Development Reaction into a Common Electronics Components Setup |
| Authors | Cun Qiang Zhang1,??/sup>, Yoo Min Park1,??/sup>, Dokyung Yang1, Tae Hyeon Yoo1,* & Hyun C. Yoon1,* |
| Institutions | 1Department of Molecular Science & Technology, Ajou University, Suwon, Republic of Korea ??/sup>These authors contributed equally to this work. *Correspondence and requests for materials should be addressed to H.C. Yoon ( hcyoon@ajou.ac.kr) & T.H. Yoo ( taehyeonyoo@ajou.ac.kr) |
| Abstract | Matrix metalloproteinase-2 (MMP-2) is closely related to the proliferation and invasion of various types of cancers. The protease is secreted by malignant tumor cells, thus allowing the enzyme to serve as a biomarker for cancer diagnosis. Methods have been developed to analyze MMP-2 activities; however, their applications to disease diagnosis have not been widely demonstrated yet because of the need for highend analytical equipment and labor-intensive processes. In this study, we developed an MMP-2 activity assay system by integrating an engineered autoinhibited 棺-lactamase which can be activated by MMP-2 in an optical sensing system consisting of reassembled common electronic components, such as a laser diode, a solar cell, and a multimeter. The autoinhibited 棺-lactamase was immobilized on a polymeric biosensing channel by a polydopamine coating and self-assembled monolayer methods. In the presence of MMP-2, the immobilized autoinhibited 棺-lactamase was converted to an active form that hydrolyzed the chromogenic cephalosporin CENTA, thereby changing the substrate color from pale yellow (貫max=340 nm) to highly discernible chrome yellow (貫max=405 nm). By reading the interfered laser-light intensity, we were able to analyze MMP-2 activities precisely both with the samples pre pared in a buffer solution and also those in urine. These results suggested that the developed system can be used for the quantitative analysis of enzyme activity related to cancer diagnosis. |
| Keyword | Matrix metalloproteinase-2, Autoinhibited 棺-lactamase, Optical biosensor |
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