| Info. | Vol.11 - No.2 (2017.06.20) |
|---|---|
| Title | Microfluidic Biochips for Simple Impedimetric Detection of Thrombin Based on Label-free DNA Aptamers |
| Authors | Taemin Lim1,??/sup>, Seung Yong Lee2,??/sup>, Jeongsuong Yang3, Seung Yong Hwang2,* & Yoomin Ahn1,* |
| Institutions | 1Department of Mechanical Engineering, Hanyang University, Ansan, Gyeonggi-do, Republic of Korea 2Department of Bionano Technology, Hanyang University, and GenoCheck Co. Ltd., Ansan, Gyeonggi-do, Republic of Korea 3Corporate R&D Institute, Samsung Electro-Mechanics Co. Ltd, Suwan, Gyeonggi-do, Republic of Korea ??/sup>These authors contributed equally to this work. *Correspondence and requests for materials should be addressed to Y. Ahn (ahnym@hanyang.ac.kr) and S.Y. Hwang (syhwang@hanyang.ac.kr) |
| Abstract | This paper presents a proof-of-concept microfluidic aptamer-based sensor for thrombin pointof-care testing using electrochemical impedance spectroscopy. The disposable biosensor is composed of a polydimethylsiloxane (PDMS) channel layer over a glass substrate. The substrate surface has an Au working electrode and a Pt reference/counter electrode. In this study, human thrombin was used as a biomarker for disease diagnosis. An unlabeled aptamer specific to thrombin was immobilized on a working electrode and electrochemical impedance was measured as thrombin was injected into the biosensor. Thrombin was detected by measuring change in impedance. The proposed biochip had a detection range of 0.1-100,000 ng mL-1 for thrombin concentration and a limit of detection of 0.1 ng mL-1. The results indicated that our biochip could be an effective tool for other molecular diagnostic systems. |
| Keyword | DNA Aptamer, Microfluidics, Thrombin, Electrochemical impedance spectroscopy, Anti-thrombin aptamer |
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