| Info. | Vol.11 - No.2 (2017.06.20) |
|---|---|
| Title | Development of a 2-Chamber Culture System for Impedimetric Monitoring of Cell-cell Interaction |
| Authors | Kin Fong Lei1,2,3,??*, Meng-Tsan Tsai4,5,??/sup>, Ming-Hong Zhong1, Chia-Hao Huang1, Ngan-Ming Tsang3,6 & Ming-Yih Lee1,2,7,* |
| Institutions | 1Graduate Institute of Medical Mechatronics, Chang Gung University, Taoyuan, Taiwan 2Department of Mechanical Engineering, Chang Gung University, Taoyuan, Taiwan 3Department of Radiation Oncology, Chang Gung Memorial Hospital, Linkou, Taiwan 4Department of Electrical Engineering, Chang Gung University, Taoyuan, Taiwan 5Department of Dermatology, Chang Gung Memorial Hospital, Linkou, Taiwan 6School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan 7Department of Cardiology, Chang Gung Memorial Hospital, Linkou, Taiwan ??/sup>These authors contributed equally to this work. *Correspondence and requests for materials should be addressed to K.F. Lei (kflei@mail.cgu.edu.tw) and M.-Y. Lee (leemiy@mail.cgu.edu.tw) |
| Abstract | In cancer research, study of cell-cell interaction is important to understand tumor initiation, progression, metastasis, and therapeutic resistance. Conventionally, transwell system was adopted and cell proliferation was quantified by end-point bio-assays. The operations are labor-intensive and time-consuming while studying of the dynamic cellular responses of cell-cell interaction. Although impedance measurement was suggested to be a promising technique to monitor cellular responses, electrodes cannot be integrated into the transwell for the measurement purpose. In this work, a 2-chamber culture system incorporated with impedance measurement technique was developed to quantitatively study cell-cell interaction. The chamber was composed of 2 sub-chambers separated with a barrier. By this design, two types of cells could be independently cultured and concurrently monitored under common medium supplied. Cell-cell interaction was demonstrated by aberrant cell proliferation induced by the EGF secreted from the transfected cells cultured on another sub-chamber. Real-time and non-invasive monitoring of cell-cell interaction was successfully demonstrated. This work provides a practical solution for monitoring the dynamic cellular responses of cellcell interaction during the culture course. It is a reliable and convenient platform and facilitate more quantitative assessments in cancer research. |
| Keyword | Impedance measurement, Cell culture, Co-culture, Cell-cell interaction, Cell-based assays |
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