| Info. | Vol.11 - No.3 (2017.09.20) |
|---|---|
| Title | Design and Fabrication of Devices for Investigating Cell-sheet Stretch |
| Authors | Yang Liu1, Yoshihiro Ojima2, Masanobu Horie3, Eiji Nagamori4 & Hideaki Fujita5,6,* |
| Institutions | 1Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, 560-8531 Osaka, Japan 2Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan 3Radioisotope Research Center, Division of Biochemical Engineering, Kyoto University, Yoshida, Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan 4Department of Biomedical Engineering, Osaka Institute of Technology, 5-16-1 Ohmiya, Asahi-ku, 535-8585, Osaka, Japan 5Quantitative Biology Center, Riken, 6-2-3 Furuedai, Suita, 565-0874 Osaka, Japan 6WPI, Immunology Frontier Research Center, Osaka University, 1-3 Yamadaoka, Suita, 565-0871 Osaka, Japan *Correspondence and requests for materials should be addressed to H. Fujita (fujita@ifrec.osaka-u.ac.jp) |
| Abstract | Recently, many efforts have been made to investigate the cell stretch response through focal adhesions, which are usually utilized by cells cultured on elastic materials. However, not all stretch sensing is mediated through focal adhesions but from cell-cell contacts such as adherence junctions. To unveil the details of the stretch-sensing mechanism through cellcell contacts, we developed a cell-sheet extension device for visualizing dynamic changes of individual living cells induced by external mechanical stretch. A cell-sheet is an ideal observation object, as it can be comprised merely of cells without any mechanical influences from external matrices while maintaining normal cell-cell adhesions as in the in vivo situation. Two microfluidic extension devices were designed and fabricated using a silicone elastomer, which were capable of extending a cell-sheet made from Caco-2 cells up to 1.3- and 1.5-fold from its original state. Based on the obtained results, we expected that such cell-sheet extension systems can be useful tools for understanding the mechanisms of cellular stretch sensing through cell-cell contacts in living cells. |
| Keyword | Mechanobiology, Mechano-transduction, Caco-2, Cell-cell attachment, Cell stretch |
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