| Info. | Vol.12 - No.1 (2018.03.20) |
|---|---|
| Title | Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum |
| Authors | SangWook Lee1, Thomas Laurell2,5, Ok Chan Jeong3,4,* & Soyoun Kim5,* |
| Institutions | 1Department of Chemistry, The University of Tokyo, Tokyo, Japan 2Department of Biomedical Engineering, Lund University, Lund, Sweden 3Institute of Digital Anti-Aging Healthcare, Inje University, Gimhea, Republic of Korea 4Department of Biomedical Engineering, Inje University, Gimhea, Republic of Korea 5Department of Biomedical Engineering, Dongguk University, Seoul, Republic of Korea *Correspondence and requests for materials should be addressed to O.C. Jeong (memsoku@inje.ac.kr) and S. Kim (skim@dongguk.edu) |
| Abstract | A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 103 orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis. |
| Keyword | Sol-gel microarray, Macro/nano-structure silicon, Reverse-phase assay, Prostate-specific antigen, Prostate cancer |
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