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Info. Vol.12 - No.1 (2018.03.20)
Title Identification of possibility of Glycyrrhiza uralensis as an allergen by protein analysis
Authors Eun-Jin An1,2, Kang-Hoon Kim1,2, In-Seung Lee1,2, Ji Young Park1,2, Yumi Kim1,2, Woo Sang Jung2, Daeho Kwon3,* & Hyeung-Jin Jang1,2,*
Institutions 1Department of Biochemistry, Graduate School, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul 02447, Republic of Korea
2Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul 02447, Republic of Korea
3Department of Microbiology, Catholic Kwandong University, College of Medicine, Gangneung 25601, Republic of Korea
*Correspondence and requests for materials should be addressed to D. Kwon (dkwon@cku.ac.kr) and H.-J. Jang (hjjang@khu.ac.kr)
Abstract Various secondary metabolites derived from plants are useful for humans to preserve their health. For this reason, medical herbs have been consumed universally without deep research about the dangerousness of these substance. However, they can produce unexpected adverse reactions in humans. Among these side effects, allergic reactions involving human immunoglobulin E can occur due to antimicrobial or antibacterial substances in plants. Glycyrrhiza uralensis has been prescribed for the treatment of numerous diseases given its multiple types of pharmaceutical efficacy, including its antimicrobial and antibacterial activities. Therefore, we researched whether Glycyrrhiza uralensis, which has antimicrobial ability, can cause allergic reaction in humans. To test the hypothesis devised here, we conducted proteomic level experiments. We utilized SDS-PAGE using a protein extract of Glycyrrhiza uralensis, with Coomassie blue staining to confirm the protein pattern and western blotting to identify Human IgE binding proteins in Glycyrrhiza uralensis. Among the many candidate sera, these methods detected three positive sera. Moreover, an identified protein section with reactivity associated with Human IgE was analyzed by LC-MS/ MS. Our experimental data provide a basis for testing the possibility of Glycyrrhiza uralensis as an allergen by identifying the reactivity between human Immunoglobulin E and proteins of Glycyrrhiza uralensis.
Keyword IgE, Allergy-Q, herbal medicine, plant protein
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