| Info. | Vol.12 - No.2 (2018.06.20) |
|---|---|
| Title | Genome-wide Identification and Expression Analyses of RPP13-like Genes in Barley |
| Authors | Jinjin Cheng1, Hui Fan1, Lin Li2, Boyao Hu3,4, Hongyun Liu1 & Zheng Liu1,* |
| Institutions | 1College of Life Sciences, Hebei University, Baoding, P.R. China 2College of Biology, Hunan University, Changsha, P.R. China 3The Affiliated School of Hebei Baoding Normal 4No.1 High School of Baoding, Baoding, P.R. China *Correspondence and requests for materials should be addressed to Z. Liu (liuzhengxp@yeah.net) |
| Abstract | Plants have evolved a series of mechanisms to resist pathogens infection. The nucleotide-binding site and leucine-rich repeat (NBS-LRR) family contains the largest number of plant disease resistance genes in plants. Recognition of Peronospora Parasitica 13-like (RPP13-like) genes belong to this superfamily and play important roles in the resistance of various plant diseases including the downy mildew caused by Peronospora parasitica. In this study, 21 RPP13-like genes were identified in barley via bioinformatics. These genes all contained CC, NB-ARC and LRR domains. The physical and chemical properties, chromosome locations, gene structures, protein motifs, 3D protein structures, and microarray based expression dynamics of these genes, as well as their phylogenetic relationship with other plant species were analyzed. Non-expression of MLOC_19262.1 was detected without pathogen infection. When barley was inoculated with the powdery mildew pathogenic fungus, the expression of MLOC_ 19262.1 reached a very high level, suggesting that this gene is an important and promising candidate resistance gene for further study. The two RPP13-like genes, MLOC_57007.2 and MLOC_5059.1 may be involved in barley regular or abiotic stress induced physiological metabolism in specific tissues or at specific developmental stages; furthermore, these functions may be associated with specific domains. These findings provided evidence for the functional diversity of plant pathogen resistance genes and will be helpful for the future characterization of the PRR 13-like gene subfamily. |
| Keyword | Barley, RPP13-like genes, Bioinformatics, Gene expression profiling, Biotic and abiotic stresses, Physiological activity |
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