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Info. Vol.12 - No.4 (2018.12.20)
Title Detection of Bacillus Cereus Using Bioluminescence Assay with Cell Wall-binding Domain Conjugated Magnetic Nanoparticles
Authors Chanyong Park1, Minsuk Kong2, Ju-Hoon Lee3, Sangryeol Ryu2 & Sungsu Park1,4,*
Institutions 1Department of Biomedical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea
2Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea
3Department of Food Science and Biotechnology, KyungHee University, Yongin 17104, Republic of Korea
4School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea
*Correspondence and requests for materials should be addressed to S. Park (nanopark@skku.edu)
Abstract Bacillus Cereus can cause blood infections (i.e., sepsis). Its early detection is very important for treating patients. However, an antibody with high binding affinity to B. cereus is not currently available. Bacteriophage cell wall-binding domain (CBD) has strong and specific binding affinity to B. cereus. Here, we report the improvement in the sensitivity of an ATP bioluminescence assay for B. cereus detection using CBD-conjugated magnetic nanoparticles (CBDMNPs). The assay was able to detect as few as 10 colony forming units (CFU) per mL and 103 CFU per mL in buffer and blood. CBD-MNPs did not show any cross-reactivity with other microorganisms. These results demonstrate the feasibility of the ATP assay for the detection of B. cereus.
Keyword Bacillus Cereus, Cell wall-binding domain, Enrichment, Magnetic nanoparticles, ATP bioluminescence assay
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