| Info. | Vol.2 - No.2 (2008.06.20) |
|---|---|
| Title | Fabrication of Nonbiofouling Surface and Its Application to Surface Plasmon Field-Enhanced Fluorescence Spectroscopy |
| Authors | Eunkyung Kim1, Bong Soo Lee2, Hyeon-Bong Pyo3, Hyun-Woo Song3, Young-Pil Kim1, Insung S. Choi2 & Hak-Sung Kim1 |
| Institutions | 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea 2Department of Chemistry and School of Molecular Science (BK21), Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701,Korea 3BIOMEMS Team, IT Convergence and Components Laboratory,Electronics and Telecommunications Research Institute,Daejeon 305-700, Korea Correspondence and requests for materials should be addressed to H.-S. Kim (hskim76@kaist.ac.kr) |
| Abstract | Reducing the non-specific binding of proteins on a chip surface is essential for the development of biosensors with high sensitivity and specificity. We fabricated poly(oligo(ethylene glycol) methacrylate) (pOEGMA) films with various thicknesses as a nonbiofouling surface for surface plasmon field-enhanced fluorescence spectroscopy (SPFS). To accomplish a sensitive SPF signal, the thickness of the pOEGMA films was controlled by surface-initiated atom transfer radical polymerization (SI-ATRP) on gold-coated glass (Au/Glass) and gold-coated Si (Au/Si) substrates. When fluorophores immobilized on the pOEGMA films were directly excited without a plasmon effect, the fluorescence intensity displayed a linear dependency on the tested thickness of the pOEGMA films on both Au/Si (9-60 nm) and Au/Glass (8-56 nm). As a result, the surface plasmon fieldinduced fluorescence intensity on Au/Glass enabled thickness-dependent sensitivity. The SPF signal on 50-nm-thick pOEGMA films was estimated to be 100 -fold stronger than that on 13-nm-thick pOEGMA films. Since the SPFS on pOEGMA film can allow an easy conjugation of biomolecules and provide a high resistance to biofouling events, our fabricated system has a great potential as a platform for biosensors with high sensitivity. |
| Keyword | Nonbiofouling surface, Poly(oligo(ethylene glycol) methacrylate) films, Surface plasmon field-enhanced fluorescence spectroscopy, Biosensor |
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