| Info. | Vol.2 - No.3 (2008.09.20) |
|---|---|
| Title | Characterization of Novel Self Assembled Materials (SAM) for Surface Modification of Sensor Chip |
| Authors | Hyung Min Cho1, Dae Ho Jang1, Seung Kwon Lee, Su Jin Ku1, Hyun Chul Kim2, Kyu Sang Yu1 & Joong Whan Lee1 |
| Institutions | 1Research Institute, Korea Materials & Analysis Corp. (K-MAC),
Daejeon, Korea 2BioConvergence Technology Research Team, Daegu Gyeongbuk Institute of Science & Technology (DGIST), Daegu, Korea Correspondence and requests for materials should be addressed to K.S. Yu (ksyu@kmac.to) |
| Abstract | Recently, researchers have been frequently using a biosensor-based gold chip couple detection method. The gold surface is modified for various materials including self assembly monolayer (SAM), biotin, streptavidin, dextran, Prolinker, protein G, lipid bi-layer, etc. for use as a biosensor, diagnosis system. The modified gold surface can enhance the immobilization amounts, orientation, activity, and stability of the receptor, and decrease the non-specific binding. Surface plasmon resonance (SPR) is made of optical biosensors that are widely gaining recognition as a valuable tool to investigate biological interactions. The key features of SPR biosensors make this technology suitable for a wide range of applications. We have SPR system of fluidic type. Our SPR system can detect biomolecules-biomolecules or biomolecules-sensor surface interaction. A biosensor for the detection of small molecules or diagnostic markers requires high sensitivity. One of the methods for enhancing sensitivity is a surface modification to provide a bio-adaptable space. In this study, we modified the surface of a bare gold chip by using our NHS-SAM for enhancing of the detection sensitivity, and we compared its results with a commercial SAM. We also observed the antigenantibody interaction and compared its results using an SPR analyzer. |
| Keyword | SPR, Biosensor, Biochip, SAM, Surface Modification |
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