| Info. | Vol.2 - No.3 (2008.09.20) |
|---|---|
| Title | Sensitivity of Quartz Crystal Microbalance Precipitation Sensor for Paraoxon-ethyl |
| Authors | Dong-Kyung Kim1 & Namsoo Kim1 |
| Institutions | 1Food Nano-Biotechnology Research Center,Korea Food Research Institute, Seongnam 463-746, Korea Correspondence and requests for materials should be addressed to N. Kim (k9130sen@hanmail.net) |
| Abstract | A quartz crystal microbalance biosensor which measured the inhibition in enzyme activity by an organophosphorus pesticide, paraoxon-ethyl, was developed. When inhibitor concentration increased, sensor response gradually decreased owing to the inhibitionof the immobilized biocatalyst. The relationship between inhibitor concentration and percent inhibition (%I) was Y (%I)=12.48X (log10 paraoxon-ethyl)+ 122.30 (r=0.8477) at the paraoxon-ethyl concentrations of 3.63횞10-10-3.63횞10-6 M. The %I values found for newly prepared acetylcholinesterase-immobilized sensor chips in the presence of 3.63횞10-7 M paraoxon-ethyl were determined and the coefficient of variability for six measurements was found as 5.46%. |
| Keyword | QCM-precipitation sensor, Pesticide detection, Enzyme inhibition |
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