| Info. | Vol.3 - No.2 (2009.06.20) |
|---|---|
| Title | Biosensors on Array Chip by Dual-color Total Internal Reflection Fluorescence Microscopy |
| Authors | Seong Ho Kang1 & Md. Shahinul Islam1 |
| Institutions | 1Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University 664-14, Duckjin-Dong, Duckjin-Gu, Jeonju 561-756, Korea Correspondence and requests for materials should be addressed to S.H. Kang (shkang@chonbuk.ac.kr) |
| Abstract | The noteworthy progress in biosensing technology during the last few decades has paved the way of the real-time detection, rapid screening, characterization and quantification of biological samples with supreme precision. Scientists in this distinct area have made it possible to enhance biochip-based biosensors using optical transducers. This review is meant to provide an overview of biosensors based on nanoarray biochips using polymer-modified glass surfaces and prism type dual-color total internal reflection fluorescence microscopy (TIRFM). It also endeavors to provide information on how the dual-color TIRFM system has made it possible to monitor theinteractions and to detect the colocalization of two different protein molecules (Alexa Fluor짰 633 goat anti-rabbit IgG, anti-actin and Alexa Fluor짰 488 conjugated actin) at the single-molecular level. |
| Keyword | Biosensors, Nanoarray biochip, Total internal reflection fluorescence microscopy (TIRFM), Single-molecule detection |
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