| Info. | Vol.3 - No.2 (2009.06.20) |
|---|---|
| Title | Characterization of Monoclonal Antibodies against Adenylate Kinase Isozyme 3: a New Cardiac Disease Diagnostic Marker |
| Authors | Yong Goo Yoo1, Hee Jong Lee1& Hyo Joon Kim1 |
| Institutions | 1Department of Biochemistry, Hanyang university,
Sa-Dong, Sangrok-Gu, Ansan, Gyeonggi-Do, Korea Correspondence and requests for materials should be addressed to H.J. Kim (kimhj104@hanyang.ac.kr) |
| Abstract | Human mitochondrial adenylate kinase plays an important role in energy metabolism by phosphoryl transfer between adenine nucleotides. Our previous studies had strongly suggested that the serum levels of human adenylate kinase 3 (hAK3) should be a biochemical marker for the necrosis of myocytes. Also, it was reported that the expression of AK3 was induced about 3 fold in the condition of hypoxia. Acute myocardial infarction (AMI) is a disease appeared when cardiac muscle cell disrupted by hypoxia. Monoclonal antibodies (Mabs) are produced widely for use in researches and also are produced in large quantities against a plethora of antigens for use in diagnosis and sometimes in treatment. In this study, B lymphocyte hybridoma cell lines producing MAb against hAK3 were constructed and characterized. For the proper and optimal applications, antibodies were grouped and characterized according to their physicochemical properties and antigenic structural characters. Identification and compairson for the complementarity determining regions (CDRs), the avidities in terms of dissociation constants and isotypes were made for each Mabs. This Mabs are useful in protein diagnostic lab-on-a-chip technology. |
| Keyword | Adenylate kinase, Monoclonal antibody, AMI, Diagnostic marker |
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