| Info. | Vol.3 - No.3 (2009.09.20) |
|---|---|
| Title | Proteomic Approach for Discovery of Potential Biomarkers for Cd Toxicity in Rice |
| Authors | Yoon Kyung Kim1 & Mi Young Lee1 |
| Institutions | 1Department of Medical Biotechnology, SoonChunHyang
University, Asan, Chungnam 336-600, Korea Correspondence and requests for materials should be addressed to M.Y. Lee (miyoung@sch.ac.kr) |
| Abstract | We have used proteomic approaches to identify differentially expressed proteins in rice roots in response to CdCl2. More than 750 protein spots that were detected by 2-dimensional gel electrophoresis showed reproducible abundance. 29 of these proteins showed significant changes after Cd treatment. Most prominent alteration was about 5 fold increase of putative glucan endo-1,3-棺-D-glucosidase, while pathogenrelated protein, glucanase and 棺-1,3-glucanase were dramatically reduced. Newly induced glutathione-Stransferase and the increase in methione synthase, fructose-bisphosphate aldolase and triosephosphate isomerase suggest their role in defense against Cd toxicity. Cd-induced alterations in specific isozyme patterns of ascorbate peroxidase, glutathione-S-transferase and peroxidase on the activity stained gels were in accordance with those of proteomic data. |
| Keyword | Proteomics, Cd, Toxicity, Biomarker, Rice |
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