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Info. Vol.3 - No.3 (2009.09.20)
Title Electrochemical Detection of Single Nucleotide Polymorphism using Gold Electrode Arrays Modified with Thiol Linkers Synthesized by Solid-Phase Strategy
Authors Sang-Myung Lee1, Eun-Kyung Kang1, Dong-Sik Shin1, Hong-Jun Cho1 , Jung-Seok Kim2, Yon-Sik Choi3, James Jungho Pak2, Youngmi Kim Pak3& Yoon-Sik Lee1
Institutions 1School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea
2School of Electrical Engineering, Korea University, Seoul 136-713, Korea
3Department of Nanopharmaceutical and Life Sciences, Department of Physiology, College of Medicine, Kyung Hee University, Seoul 130-701, Korea
Correspondence and requests for materials should be addressed to Y.-S. Lee (yslee@snu.ac.kr)
Abstract We developed an electrochemical DNA sensor based on gold electrode arrays modified with a thiol linker synthesized by novel solid-phase strategy, and could effectively detect the aging-dependant T414G mutation of mitochondrial DNA. To electrochemically detect the single nucleotide polymorphism (SNP), the gold electrode arrays were patterned on glass wafers, and the thiol linker facilely synthesized on the solid support was assembled on the gold electrode surface. On the SAM electrode surface, three kinds of DNA probes, T414G-T probe, T414G-G probe and control R probe, were covalently immobilized. Using the cyclic voltametry (CV), we could not only discriminate between full-matched DNA and single- mismatched DNA successfully, but also confirm the effectiveness of the synthesized thiol linkers for reducing the nonspecific DNA binding.
Keyword Electrochemical DNA sensor, Gold electrode arrays, Mitochondrial DNA, Single nucleotide polymorphism, Solid-phase synthesis
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