| Info. | Vol.3 - No.4 (2009.12.20) |
|---|---|
| Title | Analysis of Antigen-antibody Interactions Using Combination of Protein Nanoarray and Atomic Force Measurement |
| Authors | Dong-Ku Kang1, Hyun Park1, In-Cheol Kang2 & Soo-Ik Chang1 |
| Institutions | 1Department of Biochemistry, Protein Chip Research Center, Biotechnology Research Institute, College of Natural Science, Chungbuk National University, Cheongju 361-763, Korea 2Department of Biological Science, Hoseo University, Asan 336-795, Korea Correspondence and requests for materials should be addressed to S.-I. Chang (sichang@cbnu.ac.kr) |
| Abstract | Protein nanoarrays containing anti-angiogenin antibody IgG were fabricated on ProLinkerTM-coated Au surface by dip-pen nanolithography (DPN). An atomic force microscope (AFM) tip coated with ProLinkerTM was modified by angiogenin. We measured the interaction force between nanoarrayed anti-angiogenin antibody IgG and immobilized angiogenin on the cantilever tip by employing tethering-unbinding method. The unbinding force between anti-angiogenin antibody IgG and angiogenin was 1029짹63 pN. These results demonstrate that combination of protein nanoarray by DPN and detection using direct force measurement can be applied to analyze protein-protein interactions in nanoscale. |
| Keyword | Protein nanoarray, Dip-pen nanolithography, Interaction force, Protein-protein interaction, Angiogenin, Anti-angiogenin antibody IgG |
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