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Info. Vol.4 - No.2 (2010.06.20)
Title Optical enzyme-linked immunosorbent assay for detection of Salmonella typhimurium
Authors Sojung Park1 , Yong Tae Kim2 & Young-Kee Kim1,3
Institutions 1Division of Chemical Engineering and Program of Bio-fusion Technology, Hankyong National University, Anseong, Gyeonggi-do 456-749, Korea
2Department of Information & Control Engineering, Hankyong National University, Anseong, Gyeonggi-do 456-749, Korea
3Research Center of Chemical Technology, Hankyong National University, Anseong, Gyeonggi-do 456-749, Korea
Abstract A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) on an immunochromatographic strip was evaluated for the measurement of Salmonella typhimurium. Two different optical signal generation methods (colorimetric and chemiluminescent) were used to develop the proposed immunostrip system. Colorimetric and chemiluminescent signals could be successfully generated using the proposed immunostrip system, and S. typhimurium could be quantitatively measured in the range of 9.2횞103 to 9.2횞106 CFU/mL within 20 min (colorimetric method) and 16 min (chemiluminescent method). The selectivity and feasibility of the proposed immunostrip was confirmed by conducting experiments with spiked samples using real river water.
Keyword Enzyme-linked immunosorbent assay, Colorimetric, Chemiluminescent, Salmonella typhimurium, Immunostrip
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