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Info. Vol.4 - No.2 (2010.06.20)
Title Simple route for the detection of Escherichia coli using quantum dots
Authors Pan Kee Bae1 , Hye-Mi So2 , Kyung Nam Kim1 , Hwa Sung You1 , Kang Sik Choi1 , Chang Hae Kim1 ,Jeong-Kyu Park1 & Jeong-O Lee1
Institutions 1NanoBio Fusion Research Center, Korea Research Institute of Chemical Technology, Jang-dong 100, Daejeon 305-343, Korea
2National NanoFab Center, Gwahak-ro 33, Daejeon 305-806, Korea
Abstract We have demonstrated a simple route for the detection of Escherichia coli using well plates with quantum dot (QD) fluorescent labels. The genetically engineered E. coli strain DH5慣, containing green fluorescent protein (GFP), was used as the model system. Two approaches were employed in the detection of E. coli. In the first method, E. coli were specifically adhered onto a streptavidin-coated 96 well plate using biotin-labeled antibodies. In the second approach, E.coli were nonspecifically adsorbed onto the surfaces of 96 well plates without the use of antibodies. Where as the fluorescence signal from GFP was not correlated with the number of E. coli cells, a linear titer dependence was found from the QD-labeled E. coli. The linear dependence of the antibodyimmobilized E. coli persisted up to high (>106cfu/mL) concentrations of E.coli. However, an 짚exponential increase in the fluorescence intensity was found due to nonspecifically bound E. coli, and the signal intensity was much higher than that of antibody-immobilized E. coli.
Keyword Escherichia coli, Detection, Quantum dots, Antibody, Well plate, Fluorescence
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