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Info. Vol.4 - No.4 (2010.12.20)
Title Analysis of genomic profile in mouse lymphoma L5178Y cells exposed to food colorant gardenia yellow
Authors Md. Mujibur Rahman1,2 & Young Rok Seo1,2
Institutions 1Department of Life Science, Dongguk University-Seoul, 26 Pil-dong 3-ga, Jung-gu, Seoul 100-715, Korea
2Department of Pharmacology, Institute for Biomedical Science (IBMS), School of Medicine, Kyung Hee University, Seoul 130-701, Korea
Correspondence and requests for materials should be addressed to Y.R. Seo ( seoyr@dongguk.edu)
Abstract Gardenia yellow is renowned for its application as natural food colorant and traditional Chinese medicine. The consumption of this ingredient has been increasing in the production of several food products and the treatment of hepatic and inflammatory diseases. Safety issue is of prime importance for the gardenia yellow consumer. To evaluate the biological responses imposed by gardenia yellow, we have performed transcriptomic analysis using microarray in mouse lymphoma L5178Y cell line to gain insight into possible mechanisms of food additive gardenia yellow by which interaction in the lymphocyte cells can not be excluded. Here, the L5178Y cells were treated with gardenia yellow at non-lethal dose (5,000 mg/L). In this paper we firstly demonstrated the gene expression profile in the L5178Y cells in response to gardenia yellow. We have identified 36 mRNAs differentially expressed in the L5178Y cells against gardenia yellow. The altered expression level of 11 genes (Cd22, Fos, Gmip, Hras1, Il4, Il7r, Il10, Ndst1, Osgin1, Pik3r2, and Tnfsf4), whose products are associated with cellular processes including immune responses, signal transduction, cell proliferation, and apoptosis, was subsequently confirmed by real-time quantative reverse transcription PCR (qRT-PCR). Using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, we have also categorized these differentially expressed genes into particular pathway in accordance with gardenia yellowmediated biological processes including immunological and inflammatory responses, oxidative stress-sensitive defense, and cell proliferation. Our array analysis experiments led to the novel discovery that gardenia yellow is capable of interfering immune system and cell proliferation at transcription level, even at non-lethal dose. Our study unraveled the virtue of gene expression profiling in food toxicogenomics in terms of providing plausible possibility on the interaction of food additive gardenia yellow in correlation to pathology. Furthermore, the identified genes could be implicated as potential biomarkers for safety evaluation and health harmful effects of the gardenia yellow.
Keyword Gardenia yellow, L5178Y, Lymphoma, Microarray, qRT-PCR, Genipin
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