Go to contents

(사)한국바이오칩학회 The Korean BioChip Society

BT+IT+NT융합시대의 리더 : 한국바이오칩학회

학술지 열람

논문 정보 자세히 보기

Info. Vol.4 - No.4 (2010.12.20)
Title Multiplex detection of pathogens using an immunochromatographic assay strip
Authors Jaenam Park1, Sojung Park1 & Young-Kee Kim1,2
Institutions 1Division of Chemical Engineering and Program of Bio-fusion Technology, Hankyong National University, Anseong, Gyeonggi-do 456-749, Korea
2Research Center of Chemical Technology, Hankyong National University, Anseong, Gyeonggi-do 456-749, Korea
Correspondence and requests for materials should be addressed to Y.-K. Kim ( kim@hknu.ac.kr)
Abstract Abstract An enzyme-linked immunosorbent assay (ELISA) was performed on an immunochromatographic strip for the multiplex detection of Salmonella typhimurium, Staphylococcus aureus, Legionella pneumophila, and Escherichia coli O157:H7. The immunostrip assay was completed within 20 min. An optical (colourimetric) signal could be successfully generated using the proposed immunostrip system to selectively detect four different pathogens. The detection ranges of the immunostrip for Salmonella typhimurium, Staphylococcus aureus, L. pneumophila, and E. coli O157:H7 were determined to be 9.2횞103 to 9.2횞105 CFU/mL, 8.7횞104 to 8.7횞106 CFU/mL, 1.3횞103 to 1.3횞105 CFU/mL, and 1.2횞105 to 1.2횞107 CFU/mL, respectively. The proposed immunostrip assay system is advantageous because of high sensitivity, inexpensive equipment, cost-effectiveness, ease of use, and ease of manufacture.
Keyword Enzyme-linked immunosorbent assay, Colourimetric, Pathogens, Multiplex detection, Immunostrip
PDF File
# 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다.
→ 전체 목록 보기

(사)한국바이오칩학회 (The Korean BioChip Society)
전화 : 070-7767-9855
전자우편 : biochip@biochip.or.kr
전화 : 070-7767-9867
전자우편 : biochip2@biochip.or.kr
주소 : (우)06130, 서울특별시 강남구 테헤란로7길 22 과학기술회관 신관 804호     대표자 : 심상준, 고유번호 : 206-82-65403
팩스 : 02-921-9856 , 웹사이트 : http://www.biochips.or.kr Copyright © The Korean BioChip Society. All Rights Reserved.