| Info. | Vol.4 - No.4 (2010.12.20) |
|---|---|
| Title | Multiplex detection of pathogens using an immunochromatographic assay strip |
| Authors | Jaenam Park1, Sojung Park1 & Young-Kee Kim1,2 |
| Institutions | 1Division of Chemical Engineering and Program of Bio-fusion
Technology, Hankyong National University, Anseong, Gyeonggi-do 456-749, Korea 2Research Center of Chemical Technology, Hankyong National University, Anseong, Gyeonggi-do 456-749, Korea Correspondence and requests for materials should be addressed to Y.-K. Kim ( kim@hknu.ac.kr) |
| Abstract | Abstract An enzyme-linked immunosorbent assay (ELISA) was performed on an immunochromatographic strip for the multiplex detection of Salmonella typhimurium, Staphylococcus aureus, Legionella pneumophila, and Escherichia coli O157:H7. The immunostrip assay was completed within 20 min. An optical (colourimetric) signal could be successfully generated using the proposed immunostrip system to selectively detect four different pathogens. The detection ranges of the immunostrip for Salmonella typhimurium, Staphylococcus aureus, L. pneumophila, and E. coli O157:H7 were determined to be 9.2횞103 to 9.2횞105 CFU/mL, 8.7횞104 to 8.7횞106 CFU/mL, 1.3횞103 to 1.3횞105 CFU/mL, and 1.2횞105 to 1.2횞107 CFU/mL, respectively. The proposed immunostrip assay system is advantageous because of high sensitivity, inexpensive equipment, cost-effectiveness, ease of use, and ease of manufacture. |
| Keyword | Enzyme-linked immunosorbent assay, Colourimetric, Pathogens, Multiplex detection, Immunostrip |
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