| Info. | Vol.1 - No.2 (2007.06.20) |
|---|---|
| Title | Integrated Circuit Bipolar Semiconductor Microchip-based Optical Detection of Biomolecules: Parallel Applications as a Miniaturized Sample Platform and a Two-dimensional Photodetector |
| Authors | Sukdeb Pal1 & Joon Myong Song1 |
| Institutions | 1Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151-742, South Korea Correspondence and requests for materials should be addressed to J.M. Song (jmsong@snu.ac.kr) |
| Abstract | Recently, our laboratory developed a photodiode array (PDA) microchip based on bipolar semiconductor technology. A 12횞12 micro array of photodiodes, an array of current amplifiers, and a photodiode element-addressing circuit were integrated into a single IC chip. Each photodiode had dimensions of 300횞300 關m2 and the photodiode-tophotodiode distance was 100 關m. The chip was successfully applied to the on-chip detection of target DNA and protein based on optical transduction detection method. This report demonstrates the compatibility of the constructed PDA chips to the onchip reaction conditions and the effects of operational parameters that may lead to false data interpretation. The chip has also been used as a two dimensional photodetector in a compact optical system to detect Escherichia coli O157 : H7 based on the combined use of ELISA and laser-induced fluorescence (LIF). This work shows that the PDA microchip system has the potential to be used as a highly compact and inexpensive alternative to CCD detection for spatially separated bio-reactors. |
| Keyword | Bipolar semiconductor microchip, On-chip assay, Enzyme-linked immunosorbent assay (ELISA), Pathogenic bacteria detection |
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