| Info. | Vol.5 - No.1 (2011.03.20) |
|---|---|
| Title | Dielectrophoretic manipulation of human chromosomes in microfluidic channels: extracting chromosome dielectric properties |
| Authors | Casper Hyttel Clausen1, Maria Dimaki1, Sonia Buckley2 & Winnie Edith Svendsen1 |
| Institutions | 1Department of Micro- and Nanotechnology, Technical University of
Denmark, Kgs. Lyngby, Denmark 2Department of Electrical Engineering, Stanford University, Stanford California 94305 Correspondence and requests for materials should be addressed to C. H. Clausen (Casper.Clausen@nanotech.dtu.dk) |
| Abstract | An investigation of the dielectric properties of polyamine buffer prepared human chromo-somes is presented in this paper. Chromosomes prepared in this buffer are only a few micrometers in size and shaped roughly like spherical discs. Dielectropho-resis was therefore chosen as the method of manipulation combined with a custom designed microfluidic system containing the required electrodes for dielec-trophoresis experiments. Our results show that although this system is presently not able to distinguish between the different chromosomes, it can provide average data for the dielectric properties of human chromosomes in polyamine buffer. These can then be used to optimize system designs for further characterization and even sorting. The experimental data from the dielectrophoretic manipulation were combined with theoretical calculations to extract a range of values for the permittivity and conductivity of human poly-amine buffer prepared chromosomes. |
| Keyword | Polyamine human chromosomes, Dielectro-phoresis, Electrophoresis, Conductivity properties,Microfluidic |
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