Info.
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Vol.5 - No.3 (2011.09.20) |
Title
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Exposure of BALB/3T3 fibroblast cells to temporal concentration profile of toxicant inside microfluidic device |
Authors
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Hyunjong Shin1,??/sup>, Sanjeev Kumar Mahto1,??/sup>, Jae-Hyun Kim2 & Seog Woo Rhee1 |
Institutions
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1Department of Chemistry, Kongju National University,
Kongju 314-701, Korea
2Department of Chemistry Education, Kongju National University,
Kongju 314-701, Korea
??/sup>These authors contributed equally to this work.
Correspondence and requests for materials should be addressed to S.W. Rhee
( jisanrhee@kongju.ac.kr) |
Abstract
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This work describes a simple method that generates temporal concentration profiles of chemicals inside a microfluidic channel and exposes cultured cells to chemicals in order to mimic the exponential clearance curve of chemicals in the human body. The device fabricated with a poly (dimethylsiloxane) (PDMS) replica mold and a glass substrate was used to create the microfluidic channels. The fluid streams inside the channels were controlled by programmable syringe pumps. By controlling the relative flow rates of the fluidic inlets using separate syringe pumps, the resulting composition of the inlets that feed the concentration generator zone can be controlled to generate temporal profiles of chemicals in the observation zone. The concentration profiles of fluorescent dye were confirmed by analyzing the time-lapse fluorescence images. Finally, a temporal concentration profile of toxic cadmium solution with exponential clearance curve was exposed to BALB/3T3 fibroblast cells confluently cultured inside a microfluidic device for 3 h, and the behavior of the cells was monitored using a time-lapse microscope. The relative concentration of reactive oxygen species inside the exposed cells was measured indirectly by using a fluorescent probe to assess the degree of cell death. The method developed in this work offers a convenient way of controlling the concentration of chemicals inside microfluidic channels, and enables time-dependent exposure of chemicals to cells and monitoring of the behavior of cells for biological research applications. |
Keyword
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Temporal concentration profile, Time-dependent exposure, Cell death, Microfluidics, Cell death |
PDF File
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