| Info. | Vol.5 - No.3 (2011.09.20) |
|---|---|
| Title | Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray |
| Authors | Hyun Kyu Yoon1, In Hyuk Chung1, Hee-Jin Kim3, Ki-O Lee4, Jun Sub Kim2, Eui-Jin Park5, Sang Yong Song5,6, Sa Ik Bang5,7, Tae Sung Kim8 & Seung Yong Hwang1,2 |
| Institutions | 1Department of Biochemistry, Hanyang University, Sa-dong,
Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea 2GenoCheck Co., LTD., Sa-dong, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea 3Department of Laboratory Medicine & Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea 4Samsung Biomedical Research Institute, Samsung Medical Center, Seoul 135-710, Korea 5Bio-Med Connect Center, Samsung Medical Center, Sungkyunkwan University, Seoul 136-710, Korea 6Department of Pathology, Samsung Medical Center, Sungkyunkwan University, Seoul 136-710, Korea 7Department of Plastic Surgery, Samsung Medical Center, Sungkyunkwan University, Seoul 136-710, Korea 8School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea Correspondence and requests for materials should be addressed to S.Y. Hwang (syhwang@hanyang.ac.kr) and T.S. Kim ( tskim@korea.ac.kr) |
| Abstract | Human Leukocyte Antigen (HLA), called human Major Histocompatibility Complex (MHC), performs the crucial function of antigen presentation in human immunity,and correctly matched HLA antigens improves survival and reduces immunological complications. Therefore, a precise HLA typing method is necessary for successful transplantation between donor and recipient. Until now, many methods have been developed for HLA typing, including serological and DNA-based methods. However, these previous methods have been somewhat limited due to their requirements for a large number of samples and complex experimental procedures. To resolve this problem, in this study, we developed rapid genotyping methods for HLA-C using an oligonucleotide microarray. To evaluate the performance of the HLA-C genotyping microarray, we analyzed 50 unrelated Korean individuals. The genotyping results obtained by the oligonucleotide microarray were identical with those of PCR-SBT and PCR-SSPs, at a low-resolution, 2-digit level. Therefore, this microarray method for genotyping HLA constitutes a rapid, simultaneous, and high-throughput method that can be used as an alternative to current methods. |
| Keyword | HLA, HLA-C genotyping, Microarray, DNA chip, Transplantation |
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