Info.
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Vol.5 - No.3 (2011.09.20) |
Title
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Fluorescence detection by miniaturized instrumentation based on non-cooled CCD minicamera and dedicated for lab-on-a-chip applications |
Authors
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Rafa흢 Walczak1
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Institutions
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1 University of Technology, Janiszewski Str. 11/17, 50-372 , Poland
Correspondence and requests for materials should be addressed to RRaaffaall WWaalclczzakak ( rafal.walczak@pwr.wroc.pl) |
Abstract
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Due to high specificity and sensitivity, fluorometric detection is one of the main detection methods applied in analytical microsystems commonly known as lab-on-a-chip (LOC). In most cases, optical instrumentation for fluorescence induction and detection is based on configuration and components ?쐀orrowed??from large laboratory instruments based on an epifluorescence microscope. As a result, the optical instrumentation surrounding lab-on-a-chip is bulky, expensive and dedicated for operation only inside laboratories. In this paper a brief discussion on fluorescence detection in lab-on-a-chip is presented. Next, novel low-cost detection instrumentation utilising noncooled CCD image sensor and semiconductor laser light source is described. The instrumentation is dedicated for operation in portable and low-cost devices for different LOC-based life science applications.
Finally, an example of application of the novel method and instrumentation co-working with lab-on-a-chip for real-time PCR detection of food pathogens is briefly
described. |
Keyword
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Lab-on-a-chip, Fluorescence, Non-cooled CCD, Real-time PCR |
PDF File
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