Go to contents

(사)한국바이오칩학회 The Korean BioChip Society

BT+IT+NT융합시대의 리더 : 한국바이오칩학회

학술지 열람

논문 정보 자세히 보기

Info. Vol.5 - No.4 (2011.12.20)
Title Visualization of enzymatic DNA extension by surface plasmon resonance imaging
Authors Jaeyeon Jung1 & Jinho Hyun2
Institutions 1Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Korea 2Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-742 Korea
Correspondence and requests for materials should be addressed to J. Hyun ( jhyun@snu.ac.kr)
Abstract The enzymatic extension of single-stranded DNA on a gold substrate was studied with surface plasmon resonance (SPR) imaging and atomic force microscopy. Extension of the DNA strands was carried out by TdT, which catalyzes the addition of different deoxynucleotidyl triphosphates (dNTPs) to the 3?? hydroxyl end of DNA. The extension rate of the DNA strands depended on the dNTPs, in the order of dATP 􀎕dTTP짚dCTP짚dGTP. The time-dependence of the DNA extension was imaged using SPR.
Keyword Microbead, Enzymatic extension, DNA, Surface plasmon resonance (SPR), Imaging
PDF File
# 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다.
→ 전체 목록 보기

(사)한국바이오칩학회 (The Korean BioChip Society)
전화 : 070-7767-9855
전자우편 : biochip@biochip.or.kr
전화 : 070-7767-9867
전자우편 : biochip2@biochip.or.kr
주소 : (우)06130, 서울특별시 강남구 테헤란로7길 22 과학기술회관 신관 804호     대표자 : 김민곤, 고유번호 : 206-82-65403
팩스 : 02-921-9856 , 웹사이트 : http://www.biochips.or.kr Copyright © The Korean BioChip Society. All Rights Reserved.