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Info. Vol.5 - No.4 (2011.12.20)
Title Visualization of enzymatic DNA extension by surface plasmon resonance imaging
Authors Jaeyeon Jung1 & Jinho Hyun2
Institutions 1Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Korea 2Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-742 Korea
Correspondence and requests for materials should be addressed to J. Hyun ( jhyun@snu.ac.kr)
Abstract The enzymatic extension of single-stranded DNA on a gold substrate was studied with surface plasmon resonance (SPR) imaging and atomic force microscopy. Extension of the DNA strands was carried out by TdT, which catalyzes the addition of different deoxynucleotidyl triphosphates (dNTPs) to the 3?? hydroxyl end of DNA. The extension rate of the DNA strands depended on the dNTPs, in the order of dATP 􀎕dTTP짚dCTP짚dGTP. The time-dependence of the DNA extension was imaged using SPR.
Keyword Microbead, Enzymatic extension, DNA, Surface plasmon resonance (SPR), Imaging
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