| Info. | Vol.6 - No.1 (2012.03.20) |
|---|---|
| Title | An universal biolinker for immobilization of protein and oligoDNA on a glass slide chip |
| Authors | Hyun Jeong Kim1,??/sup>, Sang Wook Oh2,??/sup>, Sung Joong Kim3, Afsaneh Amiri1,??/sup>, Hong Tao Wang1 & Eui Yul Choi1,3 |
| Institutions | 1Department of Biomedical Science, Hallym University,
Chuncheon 200-702, Korea 2Department of Biology Education, Institute of Fusion Science and Science Education, Chonbuk National University, Jeonju 561-756, Korea 3Boditech Med Inc. Chuncheon 200-702, Korea ??/sup>These authors contributed equally to this work. ??/sup>Present address; Department of Chemistry, Islamic Azad University, Central Tehran Branch, Tehran, Iran Correspondence and requests for materials should be addressed to E.Y. Choi (euichoi@hallym.ac.kr) |
| Abstract | We have previously shown that a Calixarene derivative was an excellent agent for protein immobilization on a solid phase. We further characterized the recognition mechanism between the ?쁥ost??Calixarene derivative and ?쁤uest??molecules in this study. The rate of complex formation of Calixcrown with amino acids informed that Calixcrown had the strongest interaction with Arg among the tested amino acids. In order to confirm that Arg is an effective residue in immobilization of protein on Calixcrown-coated glass, several human recombinant superoxide dismutase tagged with different numbers of Arg or Lys were generated. The recombinant protein tagged with longer Arg was immobilized better than the ones tagged with shorter Arg or Lys. We also tested if Calixcrown can immobilize oligoDNA as efficiently as it does protein. Calixcrown most strongly captured homo-dGTP oligoDNA among the four homo-oligoDNAs. When Calixcrown chip was applied to detect single nucleotide polymorphism, it differentiated one base pair mismatch of the double helix oligoDNA. Thus, Calixarene derivative linker molecule can be used as an efficient immobilization agent for both protein and oligoDNA with the right orientation on a solid phase. |
| Keyword | Calixarene derivative, OligoDNA, Oriented immobilization, Protein chip, SNP |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
