Info.
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Vol.6 - No.2 (2012.06.20) |
Title
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Lipopolysaccharide-mediated protein expression profiling on neuronal differentiated SH-SY5Y cells |
Authors
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Nando Dulal Das1, Mi Ran Choi1, Kyoung Hwa Jung1, Ji Hyun Park1, Hyung Tae Lee1, Seung Hyun Kim2 & Young Gyu Chai1 |
Institutions
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1Division of Molecular & Life Science, Hanyang University, Ansan 426-791, Korea
2Hanyang Cell Therapy Center, College of Medicine, Hanyang University, Seoul 133-792, Korea
Correspondence and requests for materials should be addressed to Y.G. Chai (ygchai@hanyang.ac.kr) |
Abstract
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Neuroinflammation can contribute to neuronal dysfunction, death and several neurodegenerative diseases, including Alzheimer?셲 disease, Parkinson?셲 disease, amyotrophic lateral sclerosis, and multiple sclerosis. Lipopolysaccharide (LPS)-induced neuroinflammation severely affects neurons and can contribute to neuronal dysfunction and degeneration by causing the release of inflammatory and neurotoxic factors. We evaluated the long-term effects of treating differentiated SH-SY5Y cells with LPS to mimic LPS-induced neuroinflammation. Using matrix assisted laser desorption ionization-time of flight mass spectrometry and MetaCore pathway analysis software (GeneGo), the proteomic expression profiles of differentiated SH-SY5Y cells after LPS treatment was studied to determine the inflammatory effects on the process of SH-SY5Y differentiation. Long-term LPS treatment resulted in the upregulation of phosphodiesterase 4B (PDE4B), slit robo GTPase (SRGAP2), transcription repressor E2F-6, vimentin, and 70 kDa heat shock protein 9 (Mortalin/HSPA9). Taken together, our results suggest that LPS-treated differentiation of SH-SY5Y cells can lend insight into the multiple pathways involved in neurological diseases. |
Keyword
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SH-SY5Y cells, Lipopolysaccharide, Meta-Core pathway analysis software (GeneGo), Neuroinflammation |
PDF File
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