| Info. | Vol.6 - No.2 (2012.06.20) |
|---|---|
| Title | Photoactivated immobilization of single-stranded DNAs on a psoralen-functionalized surface under low pH conditions |
| Authors | Junhyoung Ahn1,2,??/sup>, Ju Young Byun3,4,??/sup>, Yong-Beom Shin1,2 & Min-Gon Kim4,5 |
| Institutions | 1Nanobiotechnology Major, School of Engineering, University of Science & Technology (UST), Daejeon 305-806, Korea 2Immune Therapy Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 305-806, Korea 3Department of Fine Chemical Engineering & Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea 4Advanced Photonics Reseach Institue, Gwangju Institute of Science & Technology, Gwangju 500-712, Kroea 5Department of Photonics & Applied Physics, Gwangju 500-712, Kroea ??/sup>These authors contributed equally to this work. Correspondence and requests for materials should be addressed to M.-G. Kim (mkim@gist.ac.kr) |
| Abstract | Single stranded DNA was photo-patterned on a psoralen-functionalized surface and conjugated with a psoralen-modified enzyme. Photoreactions between the single-stranded DNA and psoralen-functionalized materials were found to be significantaly influenced by pH, showing high efficientcy only under acidic conditions. Specifically, the photopattering of single-stranded DNA probes, such as polythymine, polyadenine, polycytosine, and polyguanine, on a psoralen-functionalized amino-dextran surface under various pH conditions (pH 3.36-8.80) showed that the polythymine probes had the highest immobilization efficiency at pH 3.36. The effect of pH was further confirmed by photopatterning the single-stranded DNA probes using the photomask. While almost no pattern was observed on the psoralen surface at pHs greater than 5.5, much more visible patterns were observed as the pH was decreased below 5.5. As a result of the irradiation of polyT15 and polyA15-labeled DNA probes, the photopatterns formed by the irradiation of polythymine probes were found to be associated with the cross-linking between the thymine and the psoralen moieties. Finally, additional studies showed that psoralen could be used as the reactive center in the photo-induced cross-linking reaction between DNA and the model protein, horseradish peroxidase (HRP). |
| Keyword | DNA immobilization, Psoralen, pH effect, DNA photopatterning, DNA-protein photocrosslinking |
| PDF File |
 # 2010년도 발행분 부터는 Springer 의 BioChip Journal 페이지에서 전문을 열람하실 수 있습니다.
# 학회회원 로그인 후 [ Springer BioChip Journal 열람하기 ] 버튼을 클릭하시면 새창으로 열립니다. |
