| Info. | Vol.6 - No.3 (2012.09.20) |
|---|---|
| Title | Quantitative lateral-flow immunoassay for the assessment of the cartilage oligomeric matrix protein as a marker of osteoarthritis |
| Authors | Sung Yub Hong1, Yoo Min Park1, , Yo Han Jang1, , Byoung-Hyun Min1,2& Hyun C. Yoon1, |
| Institutions | 1Department of Molecular Science & Technology, Ajou University,
Suwon 443-749, Korea 2Department of Orthopedic Surgery, School of Medicine, Ajou University, Suwon 443-749, Korea Correspondence and requests for materials should be addressed to H.C. Yoon(hcyoon@ajou.ac.kr) |
| Abstract | This study reports a simple and quantitative analytical method for detecting cartilage oligomeric matrix protein(COMP) using a lateral-flow immunoassay(LFI) format. An immuno-chromatographic assay was developed using a gold nanoparticle(AuNP)-conjugated monoclonal antibody(Ab) probe for the quantitative detection of COMP in human synovial fluid(SF). The detection antibody was conjugated with the AuNP (~30nm) to enable detection, and the capture antibody was immobilized within a multi-spot pattern in the test zone of a membrane strip. COMP in the human SF binds to the AuNP-conjugated detection antibody, and the COMP-Ab complex then binds to the capture antibody. The immunoassay test can be analyzed by visualizing the test strip with a commercial scanner and an imaging software program. The color density of the multi-spot pattern at the test zone increased in proportion to the concentration of COMP. The developed immunoassay exhibited a dynamic detection range from 0.6 to 20關g/mL of COMP. The detection limit of the proposed LFI system was approximately 0.2關g/mL. The test results showed good correlation with those obtained using a conventional enzyme-linked immunosorbent assay(ELISA) to detect COMP. We expect that the proposed immunoassay method, based on the quantification of COMP, is suitable for osteoarthritis diagnosis using SF samples. |
| Keyword | Cartilage oligomeric matrix protein, Lateral-flow immunoassay, Osteoarthritis, Synovial fluid, Quantitative rapid kit |
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