| Info. | Vol.1 - No.3 (2007.09.20) |
|---|---|
| Title | Thin Film Fabrication of Electroactive Protein with Heme Group |
| Authors | Sang-Uk Kim1, Young Jun Kim1, Jeong-Woo Choi1,2 & Byung-Keun Oh 1,2 |
| Institutions | 1Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Shinsoo-Dong, Mapo-Gu, Seoul 121-742, Korea 2Department of Chemical & Biomolecular Engineering, Sogang University, 1 Shinsoo-Dong, Mapo-Gu, Seoul 121-742, Korea Correspondence and requests for materials should be addressed to B.-K. Oh (bkoh@sogang.ac.kr) |
| Abstract | Horse heart myoglobin is a member of the metalloproteins, and harbors iron-containing porphyrins in its active site. Myoglobin evidences redox properties, and can transfer electrons from one site to other sites. These molecules can be used in bioelectronic applications, when fabricated as a self-assembled bio-film. We utilized 11-mercaptoundecanoic acid (11-MUA) as a chemical linker in order to immobilize myoglobin on gold (Au) substrates. The immobilization of the protein and the surface morphology of the thin film were confirmed via surface plasmon resonance (SPR) and atomic force microscopy (AFM), respectively. The results indicated that the proposed immobilization technique should prove useful for the fabrication of high-quality protein film, and this technique could be applied to the fabrication of nanoscale bioelectronics. |
| Keyword | Horse heart Myoglobin, Self assembly (SA), Metalloprotein, Surface plasmon resonance (SPR), Atomic force microscopy (AFM), Cyclic voltammetry (CV), Bioelectronics |
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