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(사)한국바이오칩학회 The Korean BioChip Society

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Info. Vol.6 - No.4 (2012.12.20)
Title Development of DNA chip for verification of 25 microalgae collected from southern coastal region in Korea
Authors Gunsup Lee1, So Yun Park2, Seungshic Yum1, Seonock Woo1, Youn-Ho Lee3, Seung Yong Hwang4, Heung-Sik Park5, Sang Hyun Moh6, Sukchan Lee7 & Taek-Kyun Lee1
Institutions 1South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 656-830, Korea
2Medical Research Center of Neural Dysfunction, Gyeonsang National University, Jinju 660-751, Korea
3Marine Living Resources Research Department, Korea Institute of Ocean Science and Technology, Ansan, Gyeonggi-do 425-600, Korea
4Department of Biochemistry, Hanyang University & GenoCheck Co. Ltd., Sangnok-gu, Ansan, Gyeonggi-do 426-791, Korea
5Korea South Pacific Ocean Research Center, Korea Institute of Ocean Science and Technology, Ansan, Gyeonggi-do 425-600, Korea
6Bio-FD&C., Ltd, Anti-aging Research Institute, 57 Eunbong-ro, Namdong-gu, Incheon 405-849, Korea
7Department of Genetic Engineering, Sungkyunkwan University, 300 Chunchun-dong, Jangan-gu, Suwon 440-746, Korea
Correspondence and requests for materials should be addressed to T.-K. Lee ( tklee@kiost.ac)
Abstract Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA barcoding analysis, i.e., DNA chip technology, is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, the diversity and phylogenetic differences among different microalgae were analyzed. Additionally, a microalgal species-specific probe was screened by 21-23 bp and the result was printed on silylated slide for use in a robotic microarrayer. As a result, we performed a DNA chip assay for each of 25 microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species.
Keyword Microalgae, DNA barcoding, Mitochondrial cytochrome c oxidase subunit I, DNA chip assay, Marker gene
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