| Info. | Vol.7 - No.1 (2013.03.20) |
|---|---|
| Title | A cross-contamination-free SELEX platform for a multi-target selection strategy |
| Authors | SangWook Lee1, Jeehye Kang2, Shuo Ren2, Thomas Laurell2,3, Soyoun Kim2 & Ok Chan Jeong4 |
| Institutions | 1Bioengineering Lab, Riken Institute, Saitama 351-0198, Japan 2Department of Biomedical Engineering, Dongguk University, Pil-dong 3-ga, Seoul 100-713, Korea 3Department of Measurement Tech. & Ind. Electrical Eng, Lund University, PO-Box 118, S221-00, Lund, Sweden 4Department of Biomedical Engineering, Inje University, 607 O-bang dong, Gimhea 621-749, Korea |
| Abstract | Multi-target aptamer selection, known as multiplex systematic evolution of ligands by exponential enrichment (SELEX), is rapidly drawing interest because of its potential to enable high-speed, highthroughput aptamer selection. The parallelization of chemical processes by integrating microfluidic unit operations is a key strategy for developing a multiplex SELEX process. One of the potential problems with on-chip multiplexing chemical processes is crosscontamination. In order to avoid this, we propose a microfluidic network platform that uses pneumatic valves to allow the serial loading and incubation of aptamers with sol-ge entrapped target proteins. After target binding inside the sol-gels, the cross contamination-free parallel elution of specifically bound aptamers is performed. The platform allows selective binding with five different targets immobilized in sol gel spots. When eluting bound species, cross-contamination is avoided by sealing the adjacent elution chambers from each other using the pneumatic microvalves. Consequently, we demonstrate specific aptamer binding to the respective protein target and subsequent aptamer elution without any cross-contamination. This proof of concept opens the way to increased automation and microscale parallel processing of the SELEX methodology. |
| Keyword | Microfluidic pneumatic valve, Microheater, Multiplex SELEX, Sol-gel immobilization, Aptamer |
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